Alzheimer's Progenitor Amyloid-beta Targets and Dissolves Microbial Amyloids and Impairs Biofilm Function

Alzheimer's disease (AD) is a leading form of dementia where the presence of extra-neuronal plaques of Amyloid-.. (A..) is a pathological hallmark. However, A.. peptide is also observed in the intestinal tissues of AD patients and animal models. In this study, it is reported that A.. monomers can target and disintegrate microbial amyloids of FapC and CsgA formed by opportunistic gut pathogens, Pseudomonas aeruginosa and Escherichia coli, explaining a potential role of A.. in the gut-brain axis. Employing a zebrafish-based transparent in vivo system and whole-mount live-imaging, A.. is observed to diffuse into the vasculature and subsequently localize with FapC or CsgA fibrils that were injected into the tail muscles of the fish. FapC aggregates, produced after A.. treatment (Fa..), present selective toxicity to SH-SY5Y neuronal cells while the intestinal Caco-2 cells are shown to phagocytose Fa.. in a non-toxic cellular process. After remodeling by A.., microbial fibrils lose their native function of cell adhesion with intestinal Caco-2 cells and A.. dissolves and detachs the microbial fibrils already attached to the cell membrane. Taken together, this study strongly indicates an anti-biofilm role for A.. monomers that can help aid in the future development of selective anti-Alzheimer's and anti-infective medicine.