Evaluation of Loop-Mediated Isothermal Amplification (LAMP) for Rapid Detection of Campylobacter jejuni
bioRxiv (Cold Spring Harbor Laboratory)(2023)
摘要
In recent years, the worldwide incidence of diarrheal diseases caused by Campylobacter jejuni has been increasing, causing large-scale outbreaks in developing countries, which added to the capacity of the microorganism to cause Guillain-Barré syndrome (GBS), increasing the need for rapid and timely diagnosis to stop and prevent outbreaks. In this study, a loop-mediated isothermal amplification (LAMP) assay based on the cdtC gene was developed and evaluated for the rapid detection of C. jejuni , using 91 strains for the standardization and validation. The LAMP assay was compared to whole-genome sequencing as the gold standard. The LAMP assay for C. jejuni showed a sensitivity of 100% (CI: 90.94 - 100%), a specificity of 100% (CI: 89.56 - 100%), a positive predictive value of 100% (CI: 90.94 - 100%) and a negative predictive value of 100% (CI: 89.56 - 100%). The assay demonstrated strong agreement between the LAMP assay and genomic sequencing (kappa value = 1). The LAMP assay based on the cdtC gene is a method that provides reliable and rapid results, with high sensitivity and specificity for the identification of C. jejuni , and is considered a suitable alternative for the diagnosis of diarrheal infections by this pathogen in low-income countries.
### Competing Interest Statement
The authors have declared no competing interest.
* BLAST
: Basic Local Alignment Search Tool
CI
: Confidence interval
DNA
: Deoxyribonucleic acid
GBA
: GenBank Access
GBS
: Guillain-Barré syndrome
INS
: Instituto Nacional de Salud
LAMP
: Loop-mediated isothermal amplification
LoD
: limit of detection
NC
: Negative control
NCTC
: National Collection of Type Cultures
NTC
: Not template control
ST
: Sequence Type
WGS
: Whole-genome sequencing.
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关键词
isothermal amplification,rapid detection,loop-mediated
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