[Characteristics and phylogenetic analysis of chloroplast genome of a new type of fruit Rubus rosaefolius ].

Yongfei Wu, Xuelian Yang, Xia Wang, Li Yan,Wanping Zhang

Sheng wu gong cheng xue bao = Chinese journal of biotechnology(2023)

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Abstract
The genomic DNA of was extracted and sequenced by Illumina NovaSeq platform to obtain the complete chloroplast genome sequence, and the sequence characteristics and phylogenetic analysis of chloroplast genes were carried out. The results showed that the complete chloroplast genome of the . was 155 650 bp in length and had a typical tetrad structure, including two reverse repeats (25 748 bp each), a large copy region (85 443 bp) and a small copy region (18 711 bp). A total of 131 genes were identified in the whole genome of . chloroplast, including 86 protein coding genes, 37 tRNA genes and 8 rRNA genes. The GC content of the whole genome was 36.9%. The genome of . chloroplast contains 47 scattered repeats and 72 simple sequence repeating (SSR) loci. The codon preference is leucine codon, and the codon at the end of A/U is preferred. Phylogenetic analysis showed that . had the closest relationship with . , followed by . and . . The chloroplast genome characteristics and phylogenetic analysis of . provide a theoretical basis for its genetic diversity research and chloroplast development and utilization.
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Key words
Rubus rosaefolius,chloroplast genome,repetitive sequence,codon bias,phylogenetic analysis
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