CFTR dysfunction in smooth muscle drives TGF & beta; dependent airway hyperreactivity

BackgroundThe primary underlying defect in cystic fibrosis (CF) is disrupted ion transport in epithelia throughout the body. It is unclear if symptoms such as airway hyperreactivity (AHR) and increased airway smooth muscle (ASM) volume in people with CF are due to inherent abnormalities in smooth muscle or are secondary to epithelial dysfunction. Transforming Growth Factor beta 1 (TGF & beta;) is an established genetic modifier of CF lung disease and a known driver of abnormal ASM function. Prior studies have demonstrated that CF mice develop greater AHR, goblet cell hyperplasia, and ASM hypertrophy after pulmonary TGF & beta; exposure. However, the mechanism driving these abnormalities in CF lung disease, specifically the contribution of CFTR loss in ASM, was unknown.MethodsIn this study, mice with smooth muscle-specific loss of CFTR function (Cftr(fl/fl); SM-Cre mice) were exposed to pulmonary TGF & beta;. The impact on lung pathology and physiology was investigated through examination of lung mechanics, Western blot analysis, and pulmonary histology.ResultsCftr(fl/fl); SM-Cre mice treated with TGF & beta; demonstrated greater methacholine-induced AHR than control mice. However, Cftr(fl/fl); SM-Cre mice did not develop increased inflammation, ASM area, or goblet cell hyperplasia relative to controls following TGF & beta; exposure.ConclusionsThese results demonstrate a direct smooth muscle contribution to CF airway obstruction mediated by TGF & beta;. Dysfunction in non-epithelial tissues should be considered in the development of CF therapeutics, including potential genetic therapies.