Polyphenol-compounds From Green Synthesis of Antimicrobial property of Silver Nanoparticles using Eichhornia crassipes : Characterization and Applications

In this study, the authors aimed to expand a sustainable and gainful methodology for the purpose of synthesizing silver nanoparticles (AgNPs) using polyphenol bioactive compounds extracted from Eichhornia crassipes. The eco-friendliness of this method was also emphasized. The ethanol leaf extract with silver nitrate solution heated and filtered then the AgNPs produced were extensively the samples were thoroughly nanoparticles charaterized using a range of analytical techniques, including UV (nanoparticles wavelength measured), FTIR (functional groups), SEM (morphology, size, range, etc.,), TEM (morphological size of nanoparticles), XRD (crystaline sturcture), EDX (elemental composition), and AFM (irregular shape). In results the characterization process revealed the presence of secondary metabolites, total phenol content (TPC) observed that the highest value of 212.47 ± 7.07 (GAE). Additionally, through GC–MS analysis 12 major bioactive compounds were identified; In UV-Visible spectrum analysized its confirmed that size of nanoparticles in 448 nm absorption of the surface Plasmon resonance band by observed. The dimension of AgNPs was analyzed through SEM was found 11 nm. TEM and SAED analyses revealed the high crystallinity of AgNPs with homogeneous polycrystalline components and lattice spacing at 0.295. XRD patterns displayed four Bragg reflections at 38.45 (111), 46.35 (200), 64.75 (220), and 78.05 (301). Energy synthesis in EDX was observed in 8.79 nm. The AFM analysis the irregular shapes noticed. The synthesized AgNPs tested antimicrobial properties against various strains of microorganisms, including Staphylococcus aureus and Fusarium graminium . The synthesized AgNPs demonstrated potent antimicrobial activity, as evidenced by the highest zones of inhibition observed against Staphylococcus aureus (16.8 ± 0.03 mm) bacteria and Fusarium graminium (12.01 ± 0.01 mm) fungi at a concentration of 80 µg/mL. Our findings concluded that polyphenolic compounds are mostily in high amount reported in fresh fruits and vegetables so for that its having biological properties, same polyphenolic compounds we identified from leaf ethanolic extract of E. crassipes .