Expression and in vitro function of anti-PD-L1 human antibody expressed in plant
PLANT BIOTECHNOLOGY REPORTS(2023)
摘要
Plants can be used to produce recombinant functional proteins such as antibodies and vaccines. In this study, transgenic plants were utilized to express anti-human programmed death ligand-1 (PD-L1) monoclonal antibodies (mAb P PD-L1) tagged with the endoplasmic reticulum retention motif KDEL. PCR and immunoblot analyses confirmed that mAb PD-L1 was expressed in transgenic plants. ELISA analysis confirmed the binding activity of mAb P PD-L1 to human prostate and urothelial cancer cell lines PC-3 and TCCSUP, respectively. Confocal imaging analysis demonstrated that mAb P PD-L1 had binding activity against human cancer cell lines similar to Avelumab (mAb M PD-L1). Confocal imaging analysis showed that the binding activity of mAb P PD-L1 to the human tonsil is comparable to mAb M PD-L1. These results suggest that mAb P PD-L1 shows functional binding activities for PD-L1 + cancer cells. Hence, this study showed that tobacco plants can be used to produce functional anti-human PD-L1 mAbs.
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关键词
Anti-PD-L1 antibodies,Avelumab,Immune system,Plant expression system,Therapeutic protein
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