Determination of α-Chymotrypsin-Catalyzed Reaction Rates at Different Temperatures and PH Values by Raman Spectroscopy

I. A. Balakhnina, N. N. Brandt, A. A. Mankova,A. Yu. Chikishev,I. G. Shpachenko

Journal of Applied Spectroscopy（2021）

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摘要

The rates of an enzymatic reaction are determined using spontaneous Raman spectroscopy. Time dependences of integrated Raman intensities in spectra of the reaction mixtures are analyzed. The rates of the α-chymotrypsincatalyzed reaction are determined at different temperatures and pH values.

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关键词

Raman spectroscopy,enzymatic reaction rate,enzyme kinetics,activity

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