Preparation of a Trp-BODIPY fluorogenic amino acid to label peptides for enhanced live-cell fluorescence imaging

Lorena Mendive-Tapia,Ramon Subiros-Funosas, Can Zhao,Fernando Albericio,Nick D Read,Rodolfo Lavilla,Marc Vendrell

Nature Protocols（2017）

引用 33|浏览1

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摘要

Fluorescent peptides with excellent target specificity are useful imaging probes. This protocol describes the synthesis of a tryptophan-based fluorogenic amino acid (Fmoc-Trp(C 2 -BODIPY)-OH) and its incorporation into peptides for live-cell imaging.

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