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Bio-orthogonal labeling as a tool to visualize and identify newly synthesized proteins in Caenorhabditis elegans

Milena Ullrich, Vanessa Liang,Yee Lian Chew,Samuel Banister,Xiaomin Song,Thiri Zaw,Hong Lam,Slavica Berber,Michael Kassiou,Hannah R Nicholas,Jürgen Götz

Nature Protocols（2014）

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Abstract
The authors of this protocol describe an approach that enables distinguishing de novo –synthesized from pre-existing proteins in the model invertebrate Caenorhabditis elegans , as a way to determine the effect of internal and external stimuli.
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