Analysis of the complete plastomes of Albizia kalkora (Roxb.) Prain 1897 (Fabaceae).

Mitochondrial DNA. Part B, Resources(2023)

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Abstract
(Roxb.) Prain 1897, belonging to the family Fabaceae, is not only a landscape tree but also a medicinal plant. At present, few plastomes have been reported from , which delays the in-depth phylogenomic studies and the development of high-resolution discriminating markers for this genus. Herein, we sequenced the first plastome of by NGS technology. The genome is a circular structure (176,158 bp), containing a large single-copy (LSC) region (91,521 bp), a small copy (SSC) region (5237 bp), and two inverted repeat (IR) regions (39,700 bp each). It has 35.45% GC content and encodes 109 unique genes, which are 76 protein-coding, 4 rRNA, and 29 tRNA genes. The genetic distance analysis of the intergenic spacer regions for , and shows four intergenic regions with very high K2p values, namely, A-D (15.04), tK-16 (10.77), 11-36 (17.63) and 3-19 (20.08), which can discriminate the three species. In addition, we identified ten pairs of regions that could be utilized to design primers to discriminate the three species. The phylogenetic analysis showed was closely related to . The results in this study will provide valuable information to elucidate the classification, identification and evolutionary history of .
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Key words
Albizia kalkora, DNA barcode, fabaceae, plastome, phylogeny
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