Upgraded D22 SEC-SANS Setup Dedicated to the Biology Community
Journal of Applied Crystallography(2023)
摘要
Described here is the current status of the upgraded in situ size-exclusion chromatography (SEC) system implemented with the D22 small-angle neutron scattering (SANS) instrument at the Institut Laue-Langevin. Since its initial proof of principle in 2016, this SEC-SANS arrangement has been continuously requested by the user community, leading to the design of an upgraded version. A detailed description of the setup and its control is provided, and a few examples of protein structural investigations are presented, which will highlight the various possibilities and limitations of the setup to optimize experimental success.
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关键词
size-exclusion chromatography,SEC,small-angle neutron scattering,SANS,SEC-SANS
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