Influence of recombinant erythropoietin on mesenchymal stem cells proliferation and differentiation during cultivation on allogenic bone matrix

Effectiveness of mesenchymal stem cells (MSCs) proliferation and differentiation was studied after addition of medicinal product containing human recombinant erythropoietin (rhEPO) into enriched culture medium with components for osteo- and angiogenesis. Cultivation of mesenchymal stem cells under static conditions using "cocktail" of growth factors and rhEPO allowed to populate allogenic discs-scaffolds with connective tissue formations with the participation of osteoblasts and elongated multicellular structures, which formed after endothelium-like cells fusion. In the presence of rhEPO MSCs differentiates more effectively in osteogenic direction than in angiogenic.