3D imaging in two photon microscopy using deformable lenses

Two Photon Microscopy has found one of its main applications in the study of deep brain regions thanks to its intrinsic optical sectioning and deep penetration capabilities. One of the main limitations of deep 3D imaging is the arise of aberrations that can be reduced if not totally corrected using Adaptive Optics (AO). In this work we present the result of using a tunable acoustic gradient lens in combination with a multi actuator deformable lens placed in the back aperture pupil of the objective lens to correct for the sample aberrations.