Engineered Green Alga Chlamydomonas Reinhardtii As a Whole-Cell Photosynthetic Biocatalyst for Stepwise Photoproduction of H2 and Ε-Caprolactone
Green chemistry(2023)
Abstract
Photosynthetic whole-cell biocatalysts are promising platforms for direct production of solar chemicals. Here, we employed the green microalga Chlamydomonas reinhardtii (hereafter Chlamydomonas) as a heterologous host for the cyclohexanone monooxygenase (CHMO) enzyme that converts exogenously added cyclohexanone to & epsilon;-caprolactone by utilising photosynthetically produced molecular oxygen (O-2) and nicotinamide adenine dinucleotide phosphate (NADPH). In addition, the innate capability of Chlamydomonas to photoproduce molecular hydrogen (H-2) was utilised in a one-pot stepwise production of H-2 and & epsilon;-caprolactone. H-2 photoproduction catalysed by innate O-2-sensitive [Fe-Fe]-hydrogenase was facilitated by initial microoxic conditions and gradually declined due to accumulation of photosynthetic O-2. This was accompanied by the biotransformation of cyclohexanone to & epsilon;-caprolactone by the heterologous CHMO. The optimal conditions for the formation of & epsilon;-caprolactone were the presence of acetate in the medium (mixotrophia), relatively low light intensity (26 & mu;mol photons m(-2) s(-1)) and addition of a low amount of ethanol [1.7% (vol/vol)]. The latter serves as a substrate inhibitor for the innate alcohol dehydrogenase (ADH) driven formation of cyclohexanol from cyclohexanone, thereby preventing competition between CHMO and ADH for the substrate, cyclohexanone. The formation of & epsilon;-caprolactone was further improved by introducing a signal sequence at the N-terminus of CHMO that directs the enzyme to the chloroplast enriched both with photosynthetic NADPH and O-2, thus exploiting the compartmentalised nature of Chlamydomonas cell structure. This approach presents new opportunities for photosynthetic green chemicals production.
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