Systematic evaluation of antibiotic activity of a cefazolin-loaded scaffold with varying 3D printing temperatures and its application in treating osteomyelitis
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY(2023)
摘要
Osteomyelitis is one of the most common inflammatory bone diseases caused by gram-positive bacteria. Although cefazolin is widely used as an osteomyelitis antibiotic against Staphylococcus aureus, its thermo-labile properties may decrease its antibiotic activity when incorporated into bone cement, such as poly-methyl methacrylate. Thus, to fully characterize the loss of antibiotic activity during the fused deposition modeling 3D-printing processes, we systematically evaluated the antibacterial activity of a cefazolin-loaded polycaprolactone (PCL)-based scaffold at varying 3D printing temperatures against S. aureus. In vitro antibacterial activity analysis revealed that cefazolin-loaded PCL scaffolds printed between 60 degrees C and 120 degrees C could maintain the antibacterial activity, whereas the antibacterial activity of scaffolds printed between 140 degrees C and 160 degrees C was significantly decreased. In addition, the therapeutic potential of the 3D-printed, scaffolds was assessed based on serum interleukin-6 (IL-6) levels, micro-computed tomography (micro-CT), and histological evaluation using a rabbit model of S. aureus-induced chronic osteomyelitis. Our in vivo results demonstrated that the serum level of IL-6 decreased in the cefazolin-loaded PCL scaffold-transplanted group. Furthermore, micro-CT and histological analyses confirmed the decreased deformation and overgrowth of the bone. The utilization of a 3D-printable, cefazolin-loaded PCL scaffold offers a promising and distinct approach for the treatment of osteomyelitis. C 2023 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
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关键词
Osteomyelitis, 3D printing, Polycaprolactone, Cefazolin, Antibacterial activity
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