Lentibacillus daqui sp. nov., isolated from high- temperature Daqu, a starter for production of Chinese Jiang- flavour Baijiu

A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterial strain, designated ZS110521(T), was isolated from high-temperature Daqu, a starter for production of Chinese Jiang-flavour Baijiu and was characterised by polyphasic taxonomy. This novel isolate grew in the presence of 0-20 % (w/v) NaCl, at pH 6.0-9.0 and 20-50 degrees C; optimum growth was observed with 8-10 % (w/v) NaCl, at pH 7.0 and 37 degrees C. A comparative analysis of the 16S rRNA gene sequence (1460 bp) of ZS110521(T) revealed that it displayed the highest similarity to Lentibacillus populi WD4L-1(T) (95.5 %), followed by Lentibacillus garicola SL-MJ1(T) (95.4 %) and Lentibacillus lacisalsi BH260(T) (95.2 %). ANI and dDDH values between ZS110521(T) and other strains of species of the genus Lentibacillus were less than 78 and 28 %, respectively. The predominant cellular fatty acids (> 10 %) of ZS110521(T) were anteiso-C-17 : 0 (37.8 %), anteiso-C-15 : 0 (28.1 %) and iso-C-16 : 0 (15.5 %). The respiratory quinone was identified as menaquinone-7 (MK-7) and the major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The polyphasic taxonomic data and the results of chemotaxonomic analysis confirmed that ZS110521(T) represents a novel species, for which the name Lentibacillus daqui sp. nov. is proposed. The type strain of this proposed species is ZS110521(T) (=CGMCC 1.19456(T) =JCM 35213(T)).