Molecular Mechanism of Lotus Seedpod Oligomeric Procyanidins Inhibiting the Absorption of Oligopeptide-Advanced Glycation End Products

Research on advanced glycation end product (AGEs) inhibitionhasgenerally focused on food processing, but many protein-AGEs will stillbe taken. Oligopeptide (OLP)-AGEs, as the main form after digestion,will damage human health once absorbed. Here, we investigated theability of lotus seedpod oligomeric procyanidins (LSOPC) to inhibitthe absorption of the OLP-AGEs and elucidated the underlying mechanism.Our results showed that the inhibition rate of LSOPC on the absorptionof OLP-AGEs was about 50 & PLUSMN; 5.38%. 0.1, 0.2, and 0.3 mg/mL couldupregulate the expression of ZO-1 and downregulate the expressionof PepT1 and clathrin. Molecular docking showed that LSOPC could competewith the binding of OLP-AGEs to PepT1 and AP-2, thus inhibiting theabsorption of OLP-AGEs. Furthermore, the interaction of LSOPC withthe OLP-AGEs reduced the surface hydrophobicity of OLP-AGEs. It alteredthe secondary structure of the OLP-AGEs, thus weakening the affinityof the OLP-AGEs to the transporter protein to inhibit the absorptionof OLP-AGEs. Together, our data revealed potential mechanisms by whichLSOPC inhibit the absorption of OLP-AGEs and opened up new perspectiveson the application of LSOPC in reducing the increasing health risksposed by OLP-AGEs.