MiRNA-470-5p suppresses epithelial-mesenchymal transition of embryonic palatal shelf epithelial cells by targeting Fgfr1 during palatogenesis.
Experimental biology and medicine (Maywood, N.J.)(2023)
Abstract
MicroRNAs (miRNAs) have been identified as crucial modulators of gene expression and to play a role in palatogenesis. The aim of this study was to explore the potential role and regulatory mechanisms of miRNAs during palatogenesis. RNA-sequencing was performed to compare the RNA expression profiles of mouse embryonic palatal shelf (MEPS) tissue between an all-trans retinoic acid (ATRA)-induced group and control group, followed by reverse transcription-quantitative polymerase chain reaction for validation, demonstrating upregulated expression of miRNA-470-5p and downregulated expression of in the ATRA-induced group. The specific binding sites of miRNA-470-5p that potentially govern expression were predicted by miRanda and TargetScan. The relationship between miRNA-470-5p and was validated in HEK293T cells by luciferase reporter assays, confirming that miR-470-5p acts directly on the 3'-untranslated region. mRNA and FGFR1 protein levels were markedly downregulated in MEPS epithelial cells over-expressing miRNA-470-5p. Functional experiments with CCK-8, cell colony formation, and 5-ethynyl-2'-deoxyuridine (EdU) staining assays revealed that upregulated miRNA-470-5p expression could inhibit the epithelial-mesenchymal transition (EMT) of MEPS epithelial cells by targeting . These findings provide a new molecular mechanism of cleft palate formation, which can inform the development of new treatment and/or prevention targets.
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Key words
MiRNA, cleft palate, epithelium, 3 & PRIME,UTR, epithelial-mesenchymal transition, Fgfr1
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