Tris(2,2’-bipyridyl)ruthenium (II) complex as a universal reagent for the fabrication of heterogeneous electrochemiluminescence platforms and its recent analytical applications

In recent years, electrochemiluminescence (ECL) has received enormous attention and has emerged as one of the most successful tools in the field of analytical science. Compared with homogeneous ECL, the heterogeneous (or solid-state) ECL has enhanced the rate of the electron transfer kinetics and offers rapid response time, which is highly beneficial in point-of-care and clinical applications. In ECL, the luminophore is the key element, which dictates the overall performance of the ECL-based sensors in various analytical applications. Tris(2,2’-bipyridyl)ruthenium (II) complex, Ru(bpy) 3 2+ , is a coordination compound, which is the gold-standard luminophore in ECL. It has played a key role in translating ECL from a “laboratory curiosity” to a commercial analytical instrument for diagnosis. The aim of the present review is to provide the principles of ECL and classical reaction mechanisms—particularly involving the heterogeneous Ru(bpy) 3 2+ /co-reactant ECL systems, as well as the fabrication methods and its importance over solution-phase Ru(bpy) 3 2+ ECL. Then, we discussed the emerging technology in solid-state Ru(bpy) 3 2+ ECL-sensing platforms and their recent potential analytical applications such as in immunoassay sensors, DNA sensors, aptasensors, bio-imaging, latent fingerprint detection, point-of-care testing, and detection of non-biomolecules. Finally, we also briefly cover the recent advances in solid-state Ru(bpy) 3 2+ ECL coupled with the hyphenated techniques. Graphical abstract