Phomactinine, the First Nitrogen-Bearing Phomactin, Produced by Biatriospora sp. CBMAI 1333

Metabolomics analyses and improvement of growth conditionswereapplied toward diversification of phomactin terpenoids by the fungus Biatriospora sp. CBMAI 1333. Visualization of molecularnetworking results on Gephi assisted the observation of phomactindiversification and guided the isolation of new phomactin variantsby applying a modified version of chemometrics based on a fractionalfactorial design. Consequentially, the first nitrogen-bearing phomactin,phomactinine (1), with a new rearranged carbon skeleton,was isolated and identified. The strategy combining metabolomics andchemometrics can be extended to include bioassay potency, structurenovelty, and metabolic diversification connected or not to genomicanalyses.