Glucose oxidation induced pH stimuli response controlled release electrochemiluminescence biosensor for ultrasensitive detection of CYFRA 21-1.

Herein, a self on electrochemiluminescence (ECL) biosensor was constructed by pH stimuli response controlled release strategy, in which SiO2-PEI as the carrier, BSA/luminol-Ab2 as the encapsulated substance, gold nanoparticles (Au NPs) as the blocking cap, glucose as the inducer. In addition, CeO2-Au was used as catalyst, which generated more O2•- to increase the ECL signal. Under the action of voltage, the glucose was oxidized to gluconic acid, which induced the pH to decrease accordingly. Therefore, Au NPs were stimulated to fall from the surface of SiO2-PEI, releasing the BSA/luminol-Ab2 to realize self on mode. With such design, the constructed self on ECL biosensor owned an ultrasensitive detection capacity of CYFRA 21-1, showing an excellent linear relationship in the range of 0.001-100000 ng/L and 0.4 fg/mL low limit of detection (LOD). It provided an innovative idea for the biosensor construction to clinical detection of lung cancer.