Goniothalamin prevents lipopolysaccharide-induced acute lung injury and inflammation via TLR-4/NF- B signaling pathway

Gongmin Zou,Long Li, LanPing Gao, YingHong Zhou, ZeNan Ma, Zekun Hu

Journal of biochemical and molecular toxicology(2023)

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Abstract
Goniothalamin (GTN) is a natural compound isolated from Goniothalamus species. It is a potent anti-inflammatory agent. However, there is a paucity of scientific data about its toxicity. This study investigated GTN's anti-inflammatory mechanism and lipopolysaccharide (LPS)-induced lung injury in mice. Mice were distributed into four groups and injected with GTN intraperitoneally (Dosage-50 and 100 mg/kg). We analyzed the wet/dry weight ratio, infiltrated inflammatory cell count, myeloperoxidase (MPO) activity, and histopathological changes in the lung tissues of the mice. Results revealed GTN alleviated LPS-induced inflammation in mice. Western Blot and enzyme-linked immunosorbent assay techniques were used to investigate the effect of GTN on pro-inflammatory cytokines and proteins involved in the MAPK and nuclear factor-B (NF-kappa B) signaling pathways. Cytokines (macrophage migration inhibitory factor, interleukin [IL]-13, IL-6, TNF-a, and IL-1 beta) were inhibited by GTN. However, IL-10 was upregulated. Western blot analysis indicated that GTN suppressed the phosphorylation of jun N-terminal kinase, nuclear factor NF-kappa-B p65, I-kappa-B, extracellular signal-regulated kinases, NF-kappa B, and p38. GTN also suppressed the expression of TLR-4 protein, thereby, inhibiting MAPK and NF-kappa B signaling pathways. Thus, GTN can effectively prevent and cure acute lung injury.
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Key words
goniothalamin, lung injury, MAPK, NF- kappa B signaling, TLR-4
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