Seduhan daun dan buah jarak pagar (Jatropha curcas L.) untuk mengendalikan nematoda perongga akar Radopholus similis secara in vitro

Radopholus similis is a nematode that infects many plantation crops. Infected plant roots by nematodes will show necrosis symptoms, then rot due to infection of secondary pathogens. Control of R. similis that is effective, environmentally friendly, and easy to be performed by the farmers is the key to success in reducing losses by this nematode. Extract of physic nut has been recognized to be effective in controlling some important pests, but there is currently no reports of its effectiveness in controlling R. similis. This study aimed to determine the effectiveness of leaves and fruit steeping of physic nut for in vitro control of R. similis. A total of 1:10 (w/v) leaves and fruit of physic nut were steepped, then filtered by a 500 mesh sieve. Steeping was then dripped into a suspension containing 40 R. similis. Twenty-four hours after treatment, the number of dead nematodes was observed. The leaves steeping of physic nut could kill up to 70% of R. similis. Mortality of R. similis treated with steeping of physic nut seeds reached 76.25%. Both steeping applications did not cause damage to the body of nematodes, thus it is presumably that the mortality was due to toxic compounds.