38P Hormonal modulation of photodynamic therapy efficacy in breast cancer 3D spheroid culture model

Most preclinical cancer treatment studies focus on the investigation on monolayer cancer cells. However, there are significant differences in cell characteristics between monolayer cells and solid tumors, thus limiting the potential efficacy of in vitro Photodynamic Therapy (PDT) studies. Hence, PDT studies on 3D spheroids might provide insights in hormonal microenvironment. This study aims to elucidate the efficacy of hexyl-aminolaevulinic acid (H-ALA) mediated PDT on 3D spheroids in hormonal simulated microenvironment.