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A Roadmap for Three-Dimensional Analysis of the Intact Mouse Ovary.

Bikem Soygur, Mariko H Foecke,Eliza A Gaylord,Adam Fries,Jing Li,Ripla Arora,Diana J Laird

Methods in molecular biology (Clifton, N.J.)（2023）

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Abstract
Recent advances in tissue clearing methodologies have enabled three-dimensional (3D) visualization of the ovary and, consequently, in-depth exploration of the dynamic changes occurring at the single-cell level. Here we describe methods for whole-mount immunofluorescence, clearing, imaging, and analysis of whole ovarian tissue in 3D throughout murine development and aging.
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Key words
intact mouse ovary,three-dimensional
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