Fluorescent probe for the identification of potent inhibitors of the macrophage infectivity potentiator (Mip) protein of Burkholderia pseudomallei

Nicolas Julian Scheuplein,Theresa Lohr,Mirella Vivoli Vega,Dyan Ankrett,Florian Seufert,Lukas Kirchner,Nicholas J. Harmer,Ulrike Holzgrabe

SLAS Discovery（2023）

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摘要

•Synthesis of a new tracer molecule.•Robust and easy screening method for a broad range of compound activities.•FP assay validation considering limited use of starting material, DMSO tolerance, variation in incubation time and temperature.•Possibility of extension to HTP assay.

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macrophage infectivity potentiator,potent inhibitors

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