Bacteria-derived dna in extracellular vesicles of serum could become a novel diagnostic biomarker for renal cell carcinoma

You have accessJournal of UrologyCME1 Apr 2023MP04-02 BACTERIA-DERIVED DNA IN EXTRACELLULAR VESICLES OF SERUM COULD BECOME A NOVEL DIAGNOSTIC BIOMARKER FOR RENAL CELL CARCINOMA Toshihiro Uemura, Atsunari Kawashima, Kentaro Jingushi, Sassi Nesrine, Toshiki Oka, Yohei Okuda, Akinaru Yamamoto, Gaku Yamamichi, Eisuke Tomiyama, Yu Ishizuya, Yoshiyuki Yamamoto, Taigo Kato, Koji Hatano, and Norio Nonomura Toshihiro UemuraToshihiro Uemura More articles by this author , Atsunari KawashimaAtsunari Kawashima More articles by this author , Kentaro JingushiKentaro Jingushi More articles by this author , Sassi NesrineSassi Nesrine More articles by this author , Toshiki OkaToshiki Oka More articles by this author , Yohei OkudaYohei Okuda More articles by this author , Akinaru YamamotoAkinaru Yamamoto More articles by this author , Gaku YamamichiGaku Yamamichi More articles by this author , Eisuke TomiyamaEisuke Tomiyama More articles by this author , Yu IshizuyaYu Ishizuya More articles by this author , Yoshiyuki YamamotoYoshiyuki Yamamoto More articles by this author , Taigo KatoTaigo Kato More articles by this author , Koji HatanoKoji Hatano More articles by this author , and Norio NonomuraNorio Nonomura More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003215.02AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: There is no clinically established diagnostic biomarker for renal cell carcinoma (RCC) patients. This study aimed to identify a novel diagnostic biomarker using bacteria-derived DNA (b-DNA) in extracellular vesicles (EVs) of serum. METHODS: A total of 88 clear cell RCC patients underwent radical or partial nephrectomy, and 10 healthy controls were enrolled in cohort A. EVs were collected from serum just before surgery and prior to antibiotic administration, and metagenomic sequencing was performed after amplification of 16S ribosomal RNA gene. Linear discriminant analysis effect size was used to compare b-DNA from both groups (Figure 1). Significantly abundant b-DNA in serum EVs of RCC patients were used to establish an index for RCC diagnosis. A total of 32 RCC patients and 16 controls were enrolled in cohort B for validation. RESULTS: In cohort A, three kinds of b-DNA (Bacteroidia, TM7-1 and Sphingomonadales) were significantly abandant in serum EVs of RCC patients (Figure 2). In ROC analysis, the area under the curve (AUC) of each b-DNA was 0.66 (Bacteroidia), 0.68 (TM7-1), and 0.72 (Sphingomonadales), respectively. We established BTS index using three kinds of b-DNA to diagnose RCC patients, and it could distinguish RCC patients from healthy controls (AUC=0.88, sensitivity 89%, specificity 40%). Logistic regression analysis showed that BTS index was an independent factor for RCC diagnosis both in all patients (Odds ratio [OR] 5.9, 95% Confidence interval [CI] 1.3-26.1, p=0.02) and in early-stage (pT1) RCC patients (OR 6.0, 95% CI 1.2-29.7, p=0.03). Finally, also in cohort B, BTS index could distinguish RCC patients from healthy controls (sensitivity 91%, specificity 40%), and it was an independent factor for RCC diagnosis both in all patients (OR 6.0, 95% CI 1.2-28.8, p=0.03) and in pT1 RCC patients (OR 7.5, 95% CI 1.3-45.5, p=0.03). CONCLUSIONS: B-DNA in serum EVs could become a novel diagnostic biomarker for RCC. Source of Funding: This work was supported by a KAKENHI grant (22K09469, 22H03213) © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e33 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Toshihiro Uemura More articles by this author Atsunari Kawashima More articles by this author Kentaro Jingushi More articles by this author Sassi Nesrine More articles by this author Toshiki Oka More articles by this author Yohei Okuda More articles by this author Akinaru Yamamoto More articles by this author Gaku Yamamichi More articles by this author Eisuke Tomiyama More articles by this author Yu Ishizuya More articles by this author Yoshiyuki Yamamoto More articles by this author Taigo Kato More articles by this author Koji Hatano More articles by this author Norio Nonomura More articles by this author Expand All Advertisement PDF downloadLoading ...