Mp01-18 decline in sperm freeze-thaw recovery rate with delayed cryopreservation

You have accessJournal of UrologyCME1 Apr 2023MP01-18 DECLINE IN SPERM FREEZE-THAW RECOVERY RATE WITH DELAYED CRYOPRESERVATION Andre Belarmino, Daniel Colin, Stacey Kenfield, Stanton Honig, James Dupree, Mary Samplaski, Dolores Lamb, Jerrine Morris, and James Smith Andre BelarminoAndre Belarmino More articles by this author , Daniel ColinDaniel Colin More articles by this author , Stacey KenfieldStacey Kenfield More articles by this author , Stanton HonigStanton Honig More articles by this author , James DupreeJames Dupree More articles by this author , Mary SamplaskiMary Samplaski More articles by this author , Dolores LambDolores Lamb More articles by this author , Jerrine MorrisJerrine Morris More articles by this author , and James SmithJames Smith More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003212.18AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Sperm cryopreservation requires in-person visits to produce an ejaculate and immediately freeze the sample. Recently, companies began offering services for mail-in semen analysis (SA) testing and cryopreservation. However, the effects of delayed cryopreservation associated with shipping times were generally understudied. Our laboratory has continued to optimize our cryopreservation technique. This study aims to define the effect of delayed cryopreservation on the recovered total motile count (TMC) compared to standard immediate cryopreservation, using our improved methods. METHODS: Fresh semen samples were provided from healthy volunteers. Samples were diluted with 5 mL of a proprietary preservation solution, mixed, and allowed to liquify for 30 minutes. Samples were then analyzed per World Health Organization (WHO) guidelines V5 and those with abnormal concentration, volume, or motility were excluded. Samples were then divided into 3 aliquots. Aliquot #1, T0 was immediately frozen with equal part cryoprotectant. Aliquot #2, T24 was left at room temperature for 24hrs, analyzed on the SQA (sperm quality analyzer) and then frozen with cryoprotectant. Aliquot #3, T48 was left at room temperature for 48hrs, analyzed on the SQA, and then frozen with cryoprotectant. 24hrs after the third aliquot was frozen, all aliquots were thawed in an incubator at 37C for 10 minutes and analyzed on the SQA. RESULTS: Eight adult men provided a total of 26 semen samples. Baseline SA values were: volume 3.4 ml±1.2, concentration 128.2±48.7 × 106, motility 49%±7%; TMC 211±109 million. A decline in % motility was observed after specimens sat at room temperature, as well as after freeze-thaw in most individuals. The average loss from freeze/thaw in TMC at T0 was 44.0%, at T24 was 47% relative to T24 pre-freeze, and at T48 was 65% relative to T48 pre-freeze (p=0.046) (Figure 1). CONCLUSIONS: This analysis reveals a superior freeze-thaw protocol compared to our prior methods, as we continue to refine our process, and follow best practice guidelines of experts in the field. Further investigation is needed to improve the methods needed for shipping with the goal of improving the freeze-thaw recovery rate with delayed cryopreservation. Source of Funding: Fellow Health Inc. © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e9 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.Metrics Author Information Andre Belarmino More articles by this author Daniel Colin More articles by this author Stacey Kenfield More articles by this author Stanton Honig More articles by this author James Dupree More articles by this author Mary Samplaski More articles by this author Dolores Lamb More articles by this author Jerrine Morris More articles by this author James Smith More articles by this author Expand All Advertisement PDF downloadLoading ...