Cataloguing Protein Complexes In Planta Using TurboID-Catalyzed Proximity Labeling.
Methods in molecular biology (Clifton, N.J.)(2023)
摘要
Mapping protein-protein interactions is crucial to understand protein function. Recent advances in proximity-dependent biotinylation (BioID) coupled to mass spectrometry (MS) allow the characterization of protein complexes in diverse plant models. Here, we describe the use of BioID in hairy root cultures of tomato and provide detailed information on how to analyze the data obtained by MS.
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关键词
protein complexes,turboid-catalyzed
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