A novel triplex real-time PCR assay for the differentiation of lumpy skin disease virus, goatpox virus, and sheeppox virus.

Wenlong Nan,Mingxia Gong, You Lu, Jinming Li,Lin Li,Hailong Qu,Chunju Liu, Ying Wang, Faxing Wu,Xiaodong Wu,Zhiliang Wang, Yiping Chen,Daxin Peng

Frontiers in veterinary science（2023）

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Abstract

The triplex qPCR is a robust, rapid, and simple tool for identifying various types of CaPV as it can successfully distinguish LSDV, GTPV, and SPPV in one reaction. Furthermore, the assay can facilitate more accurate disease diagnosis and surveillance for better control of CaPV infection.

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Key words

Capripoxvirus, detection, qPCR, differentiation, LSDV, SPPV, GTPV

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