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幽门螺杆菌的脂质组成及其与胃组织病变损伤的关系研究

李秋兰,颜芙蓉,李彩苹,李永余, 赵越真,郑小蔚,陈津

Guide Of China Medicine（2023）

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Abstract
目的：基于液相色谱-质谱联用（LC-MS）技术从脂质组学角度探讨幽门螺杆菌（Helicobacter pylori，简称H.pylori）与胃组织病变损伤的关系。方法：收集胃疾病患者31例，对其胃窦、胃体黏膜组织块进行病理检测与H.pylori培养与细菌鉴定，采用免疫印迹法进行血清H.pylori抗体分型。用LC-MS法检测H.pylori菌体的脂质成分，采用主成分分析和正交偏最小二乘法判别方法分析两组的脂质代谢谱差异，筛选差异性脂质代谢物。结果：病理学诊断为炎症11例（无异变组）、萎缩/化生及肿瘤20例
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Key words
Lipidomics,Helicobacter pylori,Differential lipid metabolites,Gastric tissue lesions,LC-MS
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