Vitellaria paradoxa inhibits arsenic-induced toxicity in Drosophila melanogaster via the augmentation of antioxidant system.
Toxicology(2023)
Abstract
The Ethyl Acetate Fraction (EACF) of Ethanol Leaf Extract of Vitellaria paradoxa (ELVp) was assessed against Sodium Arsenite (SA)-induced toxicity in Drosophila melanogaster. The Gas Chromatography-Mass Spectrometry (GC-MS) analysis of EACF was carried out. The molecular docking of the compounds obtained from GC-MS was performed against D. melanogaster glutathione-S-transferase-2 (GST-2). Firstly, D. melanogaster (Harwich strain) was treated with EACF to determine its effect on longevity. Secondly, D. melanogaster was fed with EACF (1.0 and 3.0 mg/5 g diet) and/or SA (0.0625 mM) for 5 days. Thereafter, the ameliorative role of EACF in SA-induced toxicity was evaluated using the fly's emergence rate, locomotor activity, oxidative stress and antioxidant biomarkers. The in-silico study revealed varying degrees of binding affinity of the twelve active compounds of EACF against GST-2 which was comparable with the co-crystalized ligand (glutathione). The EACF increased the longevity of D. melanogaster by 20.0 % compared with control and ameliorated SA-induced reduction of emergence rate and locomotor performance by 178.2 and 20.5 %, respectively. Additionally, EACF ameliorated SA-induced reduction of total thiol and non-protein thiols and inhibition of catalase and GST activities (p < 0.05). These results corroborated with histological data obtained in the fat body of D. melanogaster. Overall, EACF augmented the antioxidant system of D. melanogaster and prevented sodium arsenite-induced oxidative stress due to its high antioxidant property.
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