Negative Ion-Mode N-Glycan Mass Spectrometry Imaging by MALDI-2-TOF-MS.

Methods in molecular biology (Clifton, N.J.)（2023）

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摘要

Matrix-assisted laser desorption/ionization mass spectrometry imaging with laser-induced postionization (MALDI-2-MSI) has proven a powerful tool for the in situ analysis of N-linked glycosylation, or N-glycans, directly from clinical tissue samples. Here we describe a sample preparation protocol for the analysis of N-glycans from formalin-fixed, paraffin-embedded tissue sections.

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关键词

spectrometry,imaging,ion-mode,n-glycan

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