Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain.

Marta E Álvarez Argüelles, Zulema Pérez Martínez, Susana Rojo Alba,Jose María González Alba,Ana María Fernandez-Verdugo, Isabel Costales González, Garbriel Martín Rodríguez,Jose Antonio Boga Riveiro, Mario Margolles Martins,Santiago Melón García

Emerging infectious diseases（2023）

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摘要

When a monkeypox virus outbreak began in several parts of the world in May 2022, timely and accurate diagnosis became mandatory. In our laboratory, a real-time quantitative PCR was designed and evaluated in several patient samples and compared with isolation results. Genomic viral load was related to virus viability.

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