The Impact of Upper and Lower Motor Neuron Burden on Diagnostic Certainty, and Clinical Course of Spinal-Onset Amyotrophic Lateral Sclerosis: a Cluster-Based Approach.

Upper motor neuron (UMN) and lower motor neuron (LMN) involvement represent the core clinical features of amyotrophic lateral sclerosis (ALS). Several studies divided patients into prevalent UMN and LMN impairment phenotypes to investigate the association between motor systems impairments and ALS clinical course. However, this distinction was somehow heterogeneous and significantly affected the comparability across studies. This study aimed to investigate whether patients spontaneously segregate based on the extent of UMN and LMN involvement without a-priori categorization and to identify potential clinical and prognostic features of different clusters. Eighty-eight consecutive spinal-onset ALS patients were referred to an ALS tertiary center between 2015 and 2022. UMN and LMN burden was assessed with the Penn Upper Motor Neuron scale (PUMNS) and the Devine score, respectively. PUMNS and LMN scores were normalized into 0–1 and analyzed using a two-step cluster analysis and the Euclidean distance measure. The Bayesian Information Criterion was used to determine the cluster number. Demographic and clinical variables were tested for differences among the clusters. Three distinct clusters emerged at cluster analysis. Patients in “cluster-1” showed moderate UMN and severe LMN involvement, corresponding to the typical ALS phenotype. Patients in “cluster-2” showed mild LMN and severe UMN damage, corresponding to a predominant UMN phenotype, while “cluster-3” patients showed mild UMN and moderate LMN damage, corresponding to a predominant LMN phenotype. Patients in “cluster-1” and “cluster-2” showed a higher prevalence of definite ALS than those in “cluster-3” (61