Chromatin Immunoprecipitation Assay in Mouse Retinal Tissue.

Chromatin immunoprecipitation (ChIP) is one of the most widely used methods for investigating interactions between proteins and DNA sequences. ChIP plays an important role in the transcriptional regulation study, which can locate the target genes of transcription factors and cofactors or monitor the sequence-specific genomic regions of histone modification. To analyze the interaction between transcription factors and several candidate genes, ChIP coupled with quantitative PCR (ChIP-PCR) assay is a basic tool. With the development of next-generation sequencing technology, ChIP-coupled sequencing (ChIP-seq) can provide the protein-DNA interaction information in a genome-wide dimension, which helps a lot in identifying new target genes. This chapter describes a protocol for performing ChIP-seq of transcription factors from retinal tissues.