Utilization of erythrosine B in spectrofluorimetric quenching analysis of amlodipine and perindopril in pharmaceutical and biological matrices.

A spectrofluorimetric approach, that is sensitive, simple, validated, and cost-effective has been proposed for the estimation of amlodipine (AML) and perindopril (PER) in their bulk powders, pharmaceutical formulations and spiked human plasma. The recommended approach utilized the quantitative quenching impact of the two cited drugs on the fluorescence intensity of erythrosine B as a result of binary complex reactions among each drug with erythrosine B in pH 3.5 (Teorell & Stenhagen buffer). The quenching of erythrosine B's fluorescence was recorded at 554 nm after excitation at 527 nm. The calibration curve was detected in a range of 0.25-3.0 μg mL , with a correlation coefficient of 0.9996 for AML and 0.1-1.5 μg mL , with a correlation coefficient of 0.9996 for PER. The established spectrofluorimetric approach was validated for the estimation of the cited drugs with high sensitivity regarding ICH guidelines. Thus, the established approach could be utilized for quality control of the cited drugs in their pharmaceutical formulations.