Impact of nanopore-based metagenome sequencing on tick-borne virus detection.

Koray Ergunay,Ender Dincer,Silvia A Justi,Brian P Bourke, Suppaluck P Nelson, Hsiao-Mei Liao,Mehmet Ozkan Timurkan,Bekir Oguz,Ismail Sahindokuyucu,Omer Faruk Gokcecik, Drew D Reinbold-Wasson,Le Jiang,Nicole L Achee,John P Grieco,Yvonne-Marie Linton

Frontiers in microbiology（2023）

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摘要

NS was observed to surpass broad-range and nested amplification in detection and to generate sufficient genome-wide data for investigating virus diversity. It can be employed for monitoring pathogens in tick vectors or human/animal clinical samples in hot-spot regions for examining zoonotic spillover.

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