Crucial Role of the Ca 2+ /CN Signaling Pathway in the Antifungal Activity of Seselin against Botrytis cinerea .

causes gray mold in many fruit and vegetable crops. We previously found that Seselin (SL) displayed antifungal activity against (EC = 6.1 μg·mL), and this study investigated the effects of Ca and the Ca/CN signaling pathway on its antifungal activity against . The results indicated that exogenous Ca, Cyclosporine A, and Verapamil reduced the sensitivity of SL against ; SL significantly reduced the intracellular Ca concentration in the hyphae; the sensitivity of strains Δ1 and Δ1 to SL were significantly increased; and the expressions of 1, 1, , 1, and 1 genes of the Ca/CN signaling pathway were significantly downregulated by SL treatment. Hence, SL is a potential compound for developing fungicides against . SL dramatically reduces intracellular Ca concentration and disturbs Ca homeostasis, leading to cell death. The Ca/CN signaling pathway plays an important role in the antifungal activity of SL against .