Application of a Multiplex PCR Assay for Molecular Identification of Pathogenic and Non-Pathogenic Leptospires based on lipL32 and 16S rRNA Genes.

Leptospirosis is a serious zoonotic infection and the most prevalence disease is in the tropical and subtropical region. The definitive diagnosis of Leptospirosis, caused by spirochetes of the genus infection is already using culture methods, serological tests such as the microscopic agglutination test (MAT) and molecular detection methods (PCR) are possible. In this study, we used multiplex PCR method for detection of pathogenic and non - pathogenic based on and genes. All serovars were obtained from the Reference Laboratory of Microbiology Department, Razi Vaccine and Serum Research Institute, Karaj, Iran. The PCR product for the and genes was 272 bp and 240 bp respectively. The sensitivity amplification for the multiplex assay was 10 pg / μl for gene and 10 pg / μl for gene. The sensitivity for multiplex PCR was 10 pg / μl. The results supported the idea that multiplex PCR can be used to detect samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much easily than conventional methodologies. Due to the slow growth of and the importance of time in diagnosis, molecular methods such as PCR are suggested.