The synergistic effects of PRDX5 and Nrf2 on lung cancer progressionand drug resistance under oxidative stress in the zebrafish models br

Previous studies have shown that PRDX5 and Nrf2 are antioxidant proteins related to abnormal reactiveoxidative species (ROS). PRDX5 and Nrf2 play a critical role in the progression of inflammations and tumors. Thecombination of PRDX5 and Nrf2 was examined by Co-immunoprecipitation, western blotting andImmunohistochemistry. H2O2was applied to affect the production of ROS and induced multi-resistant protein 1(MRP1) expression in NSCLC cells. The zebrafish models mainly investigated the synergistic effects of PRDX5 andNrf2 on lung cancer drug resistance under oxidative stress. We showed that PRDX5 and Nrf2 form a complex andsignificantly increase the NSCLC tissues compared to adjacent tissues. The oxidative stress improved the combinationof PRDX5 and Nrf2. We demonstrated that the synergy between PRDX5 and Nrf2 is positively related to theproliferation and drug resistance of NSCLC cells in the zebrafish models. In conclusion, our data indicated thatPRDX5 could bind to Nrf2 and has a synergistic effect with Nrf2. Meanwhile, in the zebrafish models, PRDX5 andNrf2 have significant regulatory impacts on lung cancer progression and drug resistance activities under oxidative stress