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A high-performance electrochemical biosensor using an engineered urate oxidase.

Zheng Wei,Tanja Knaus,Yuxin Liu,Ziran Zhai,Andrea F G Gargano,Gadi Rothenberg,Ning Yan,Francesco G Mutti

Chemical communications (Cambridge, England)（2023）

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Abstract
We constructed a high-performance biosensor for detecting uric acid by immobilizing an engineered urate oxidase on gold nanoparticles deposited on a carbon-glass electrode. This biosensor showed a low limit-of-detection (9.16 nM), a high sensitivity (14 μA/μM), a wide range of linearity (50 nM-1 mM), and more than 28 days lifetime.
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Key words
electrochemical biosensor,urate oxidase,high-performance
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