Hantzsch ester as efficient and economical NAD(P)H mimic for in vitro bioredox reactions.

Biocatalysis has emerged as a valuable and reliable tool for industrial and academic societies, particularly in bioredox reactions-related fields. The cost of cofactors, especially those needed to be replenished at stoichiometric amounts or more, is the chief economic concern for bioredox reactions. In this study, a readily accessible, inexpensive, and bench-stable Hantzsch ester is verified as the viable and efficient NAD(P)H mimic by four enzymatic redox transformations including two non-heme diiron N-oxygenases and two flavin-dependent reductases. This finding provides the potential to significantly reduce the costs of NAD(P)H-relying bioredox reactions.