Real-Time Portable LAMP Assay for a Rapid Detection of Xylella fastidiosa In-Field.

Early diagnosis is part of a decision-making process which in the case of plant diseases may prevent the spread of invasive plant pathogens and assist in their eradication. Significant advantages could be obtained from moving testing technology closer to the sampling site, thereby reducing the detection time. This chapter describes a portable real-time LAMP assay for a specific detection of Xylella fastidiosa in-field. The LAMP assay, including DNA extraction, allows a complete and specific in-field analysis in just 40 minutes, enabling the detection of pathogen DNA in host tissues.