Calibration of cell-free DNA measurements by next-generation sequencing.

Derek Hoerres, Qunsheng Dai,Sandra Elmore,Siddharth Sheth,Gaorav P Gupta,Sunil Kumar,Margaret L Gulley

American journal of clinical pathology（2023）

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摘要

Our novel strategy for calibrating NGS assays suggests potential for a universal reference material to overcome biological and preanalytical variables hindering traditional NGS strategies for quantifying disease burden.

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关键词

DNA sequencing, digital PCR, EBV, calibration, plasma, cell-free DNA, disease monitoring, viral load

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