Cytobacillus spongiae sp. nov. isolated from sponge Diacarnus spinipoculum .

A novel Gram-stain-positive, aerobic and motile bacterium, designated strain CY-G, was isolated from a sponge () collected from the Red Sea. The strain grew at 13-43 °C (optimum 30 °C), pH 5.5-10.0 (optimum pH 9.0) and with 0-8.0 % (w/v) (0-1.37 M) NaCl (optimum 0 %). The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that CY-G represents a member of the genus , with the highest sequence identity to H2 (97.05 %), followed by IAM 12464 (96.76 %). The major cellular fatty acids (>5 % of the total) of CY-G were Ciso, Ciso, Cω7 alcohol, C, Ciso ω10 and Ciso. The major polar lipids were glycolipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major respiratory quinone is menaquinone-7 (MK-7). The cell-wall peptidoglycan contains -diaminopimelic acid. The total genome size of CY-G is 4 789 051 bp. The DNA G+C content is 38.83 mol%. The average nucleotide identity and DNA-DNA hybridization among CY-G and type strains of other species of the genus were 76.79-78.97 % and 20.10-24.90 %, respectively. On the basis of the results of phylogenetic analysis, physiological and biochemical characterization, strain CY-G represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CY-G (=MCCC 1K06383=KCTC 43348).