A multicentre external quality assessment: A first step to standardise PCR protocols for the diagnosis of histoplasmosis and coccidioidomycosis.

Dunja Wilmes,Ferry Hagen,Cristina Verissimo,Alexandre Alanio,Volker Rickerts,Maria José Buitrago

Mycoses（2023）

Cited 0|Views16

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Abstract

All five laboratories were able to correctly detect H. capsulatum and Coccidioides spp. DNA and qPCRs had a better performance than specific cPCR and broad-range cPCR. EQAs may help standardise in-house molecular tests for the so-called endemic mycoses improving patient management.

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Key words

Coccidioides, Histoplasma, kinetic polymerase chain reaction, multicentre trial, PCR, quality control

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