NIR II light response-based PDA/AuPt@CuS composites: Simultaneous readout of temperature and pressure sensing strategy for portable detection of pathogenic bacteria

In this study, we developed a simultaneous readout of pressure and temperature dual-signals platform based on the second near-infrared (NIR II) light response-based polydopamine (PDA)-functionalized-AuPt nanoparticles (NPs)@CuS nanosheets (PDA/AuPt@CuS NS) composite. Due to the excellent NIR photothermal performance of PDA/AuPt@CuS NS, it contribute to the decomposition of H2O2 and NH4HCO3 to generate gases (including O-2, CO2, and NH3) can be promoted, which can amplify the pressure signals in a sealed container. A sandwich mode is formed between Fe3O4 NPs and PDA/AuPt@CuS NS based on the dual-aptamer when target pathogenic bacteria is present. And, it is possible to convert the molecular recognition signals between the dual-aptamers into amplified pressures and temperatures, which can be read out by a portable pressure meter and smart-phones simultaneously. It may offer the possibility for quantitative POCT analysis of Pathogenic Bacteria. Moreover, because of the high photothermal efficiency of this method, the developed dual-mode method can achieve that following the detection of bacteria and killing them immediately. As a result, secondary contamination is eliminated and bacterial transmission is avoided. The developed dual-signal sensing platform is also inexpensive, simple to operate and rapidly, indicating that it can be used for food safety analysis, clinical applications, and environmental monitoring.