Gramella oceanisediminis sp. nov., isolated from deep-sea sediment of the Indian Ocean.

A taxonomic study was carried out on strain GC03-9, which was isolated from deep-sea sediment of the Indian Ocean. The bacterium was Gram-stain-negative, catalase-positive, oxidase-negative, rod-shaped and gliding motile. Growth was observed at salinities of 0-9 % and at temperatures of 10-42 °C. The isolate could degrade gelatin and aesculin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GC03-9 belonged to the genus , with the highest sequence similarity to JCM 33424 (97.9 %), followed by KCTC 23123 (97.2 %) and other species of the genus (93.4-96.3 %). The average nucleotide identity and the digital DNA-DNA hybridization estimate values between strain GC03-9 and JCM 33424 and KCTC 23123 were 25.1 and 18.7 % and 82.47 and 75.69 %, respectively. The principal fatty acids were iso-C (28.0 %), iso-C 3OH (13.4 %), summed feature 9 (iso-C 9 and/or 10-methyl C; 13.3 %) and summed feature 3 (C 7 and/or C 6; 11.0 %). The G+C content of the chromosomal DNA was 41.17 mol%. The respiratory quinone was determined to be menaquinone-6 (100 %). Phosphatidylethanolamine, one unknown phospholipid, three unknown aminolipids and two unknown polar lipids were present. The combined genotypic and phenotypic data showed that strain GC03-9 represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain GC03-9 (=MCCC M25440=KCTC 92235).