Glutathione synthetase from Hydra vulgaris: Molecular cloning, overexpression, purification and partial characterization.

Nusrat Perween,Komal Pekhale,Gauri Haval, Gaurav Khude,Surendra Ghaskadbi,Saroj S Ghaskadbi

Protein expression and purification（2023）

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Abstract

•For the first time, Glutathione synthetase from Hydra vulgaris Ind-Pune has been cloned, expressed, and characterized.•Homology modeling indicates that it is comparable to GS in higher vertebrates.•Transcripts of HvGS were only detected in the ectoderm of whole Hydra.•Expression and enzymatic activity of HvGS were induced by exposure to H2O2.

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